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2017 Session 1 mtDNA sequences

June 9, 2017 in mitochondrial DNA

The attached zip file has ABI sequence trace files. Download the zip file and unzip. You need a program that will open ABI trace files to view and edit them. The files ending in .fa are plain text files of the DNA sequence, trimmed at the ends to remove ambiguous sequences. If no .fa file exists for a sample, the sequencing reaction for that sample did not work.

The hominid sequences file has Neanderthal and Denisovan DNA sequences for the same region of mitochondrial DNA, for comparison purposes.


(Contains 2 attachments.)

2016 Session 2 mtDNA sequences

July 15, 2016 in mitochondrial DNA


(Contains 4 attachments.)

GMO testing

July 15, 2016 in GMO

GMOGroup 1-4

GMO test – tortilla chip

GMOGroup 5-8

corn chips

GMOGroup 9-12

soybeans

GMOGroup 13-16

papaya from Guatemala

GMOGroup 17-20

Doritos – GMO positive!

2016-2 Alu insertion polymorphisms

July 15, 2016 in Alu insertion polymorphisms

Samples 17-20

Samples 17-20

Samples 13-16

Samples 13-16

Samples 9-12

Samples 9-12

Samples 5-8

Samples 5-8

Samples 1-4

Samples 1-4

2016-2 mtDNA PCR gels

July 12, 2016 in mitochondrial DNA

This session we used nanodrop to quantitate DNA. The nanodrop measurements are listed below each gel image.

mtGel 1

Gel 1: samples 1-8

Nanodrop measurements (ng/ul): 1: 25 2: 31 3:30 4:73 5:5.6 6:30 7:57 8:56

mtGel 2

Gel2: samples 9-16

Nanodrop: 9: 35, 10: 23, 11:4, 12:41, 13:9, 14:15, 15:5.5, 16:5.6

mtGel 3

samples 17-20

Nanodrop: 17: 26.5, 18: 30, 19: 9, 20: 146

2016-2 Forensic DNA Analysis Gels

July 10, 2016 in forensic DNA analysis

Group 1

Group 1

Group 2

Group 2

Group 3

Group 3

Group 4

Group 4

Group 5

Group 5

2016 Session 2 – Restriction Digests

July 7, 2016 in Restriction digests

Gel images of restriction digests, run July 7, 2016

RD-Group1

Group 1

RD-Group2

Group 2

RD-Group3

Group 3

RD-Group4

Group 4

RD-Group5

Group 5

2016 Session 1 Week 3 Photos

July 4, 2016 in environmental science

Group picture

Group picture

Group picture

Group picture

JCEC enviroscape model

JCEC enviroscape model

JCEC chemical water testing

JCEC chemical water testing

2016 Session 1 GMO test gel images

June 10, 2016 in GMO

In this session, student groups tested various foods for GMO content by PCR for 35S or NOS terminator sequences. We used BioRad’s GMO test kit. Each gel had 7 lanes:

  1. negative control (oats) with plant primers
  2. negative control (oats) with GMO primers
  3. test food with plant primers
  4. test food with GMO primers
  5. positive control DNA with plant primers
  6. positive control DNA with GMO primers
Group 1 - no result

Group 1 – no result

Group 2 - tortilla chips

Group 2 – tortilla chips

Group 3 - cornmeal

Group 3 – cornmeal

Group 4 - fresh corn

Group 4 – fresh corn

Group 5 - "GMO-free" tortilla chips from Costco

Group 5 – “GMO-free” tortilla chips from Costco

Group 6 - blue corn tortilla chips

Group 6 – blue corn tortilla chips

 

 

2016 Session 1 mtDNA sequence files

June 9, 2016 in mitochondrial DNA

The 2016 Session 1 mtDNA HV1 sequences are attached as zip files. Of the 23 samples, 11 samples returned good quality sequence data.


(Contains 4 attachments.)
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